Electrochemical Analysis

current traces and IV plots

Electrical properties of agNAT8 transporter. (A) Families of currents induced by square pulse polarization of oocyte membranes in a 98 mmol l –1 NaCl solution (left) and after addition of 2 mmol l –1 phenylalanine (right) ( V hold, holding voltage=–50 mV, first pulse= +30 mV, last pulse= –120 mV; increment = –10 mV). (B) Family of I / V plots upon ion substitution in the bathing media. Line colors and thickness represent different ion and substrate profiles, respectively (see inserts). (C) I / V profiles with subtracted leak currents in the absence of substrate molecules. The applied Phe concentrations in B and C are equal 1 mmol l –1. Return to Dmitri Boudko