Immunolocalization of transport proteins in longitudinal sections of mosquito alimentary canal from various regions. H+V-ATPase (red) and Na+/K+ P-ATPase (green) antibodies labeled sections of the gastric caeca (GC; A), anterior midgut (AMG; B), posterior midgut (PMG; C,D), a Malpighian tubule (MT; white arrow in D) and rectum (G). The apical membrane of posterior midgut region is labeled with AgNHA1 antibody (red; E) and a nutrient amino acid transporter, AgNAT8 antibody (red; F). The yellow color in A,D,G results from the colocalization of H+ V-ATPase (red) with Na+/K+ V-ATPase (green). The nuclei are labeled blue with DRAQ. Scale bar, 100 µm.